Abstract: In order to know the virulence gene expression of foodborne Listeria monocytogenes in four kinds of broth,to reveal the toxicity levels of Listeria monocytogenes in different kinds of meat products. The PCR was employed to investigate 27 virulence genes in the Listeria monocytogenes strain Lm319. The expression level of 27 virulence genes in four kinds of broth was carried out by using RT-PCR. The results showed that a total of 23 virulence genes were detected in Listeria monocytogenes strain Lm319. As for the expression of virulence gene,both the number of virulence gene and the magnitude of gene expression were the highest in pork broth,followed by mutton broth,then beef broth. The number of virulence genes which could express was the least in chicken broth. It also found that the expression level of iap,fbp,hpt and bsh related to prf A. This study indicated there was a big difference in the expression level of virulence gene when the Listeria monocytogenes grew in different environment.