基于核酸适体微球的沙门氏菌检测方法研究

Study on detection method of salmonella using aptamer- conjugated microbeads

摘要: 目的:研究基于核酸适体的微球芯片系统应用于沙门氏菌检测的可行性。方法:采用偶联有沙门氏菌适体的微球作为载体,以荧光作为检测信号建立微球芯片体系,并优化影响检测结果的各项因素。结果:通过此方法检测20份水产样品,6份检出沙门氏菌,检出率为30%。结论:建立的检测体系能实现对沙门氏菌的快速,准确的检测,且具有较高的特异性。

Abstract: Objective: To investigate the feasibility of aptamer- conjugated microbeads for the detection of salmonella.Methods: Amino- terminal aptamer probes were covalently attached onto the surface of microbeads.Fluorescence from microbeads was detected as signal source, and parameters were optimized which may affect detection of the target.Results: Salmonella were detected in six of 20 aquatic product samples by this method, and the detection rate was 30%. Conclusion: The aptamer- conjugated microbeads can be used for high throughput and accurate detection of salmonella.