指纹图谱和主成分分析法评价不同海拔高度的桔梗药材质量

Quality evaluation of Platycodon grandiflorum from different altitude by PCA and fingerprint

摘要: 目的:对不同海拔高度桔梗药材质量进行分析与综合评价。方法:采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法,Kinetex C18色谱柱（4.6mm×100mm,5μm）;流动相乙腈（A）-0.1%甲酸溶液（B）,梯度洗脱;流速0.4m L·min-1;并对其进行聚类和主成分分析。结果:建立了不同海拔高度桔梗药材的HPLC-ELSD指纹图谱,共有峰为8个,相对峰面积有不同程度的变化。聚类分析为2大类,主成分分析选出3个主因子。结论:该方法具有良好的重复性和稳定性,为有效控制桔梗药材的质量提供依据。

Abstract: Objective: To analysis and comprehensive evaluation of Platycodon grandiflorum from different altitude and evaluate its quality.Methods: HPLC- ELSD was performed using a Kinetex C18column ( 4.6mm × 100 mm, 5μm) .with gradient elution of acetonitrile ( A) -0.1% formic acid ( B) at the flow rate of 0.4m L·min- 1, and the clustering and principal component analysis. Results: The HPLC- ELSD fingerprint of Radix Platycodon grandiflorum in different altitude were established and it had 8 characteristic common peaks, the relative peak area had different degrees of change.Clustering analysis for two categorys, the principal component analysis to select 3 main factor.Conclusion:This method had good repeatability and stability, and can be used for quality evaluation control of Platycodon grandiflorum.