响应面法优化水黄芹总黄酮提取工艺及其抗氧化研究

Optimization of extracting total flavonoids from Phyla nodiflora ( L.) by response surface methodology and its antioxidant activity

摘要: 目的:确定水黄芹中总黄酮的最佳提取工艺,并评价其抗氧化活性。方法:在单因素实验基础上,利用响应面法优化水黄芹总黄酮的提取条件;通过总黄酮对DPPH·和ABTS·的清除作用来研究其抗氧化活性。结果:水黄芹总黄酮最佳提取工艺为乙醇浓度74%、料液比1∶30(g/m L)、提取温度72℃、提取时间115min,此条件下,总黄酮得率为1.59%,与预测值1.60%接近,其偏差为0.63%。水黄芹总黄酮对DPPH·和ABTS·具有较好的清除能力,其IC50值分别为7.67、12.88μg/m L。

Abstract: Objective: To explore the optimum extraction process of total flavonoids from Phyla nodiflora ( L.) and evaluate the antioxidant activity. Methods: Based on the single- factor tests, the extraction conditions of total flavonoids from Phyla nodiflora ( L.) were optimized by response surface methodology. Meanwhile, the antioxidant activity of total flavonoids was assessed by scavenging assays of DPPH· and ABTS· Results: The optimum extraction conditions were as follows: ethanol concentration 74%, liquid- solid ratio 1 ∶ 30, extraction temperature72℃ and extraction time 115 min. Under the optimal conditions, the extraction rate of total flavonoids was 1.59%, which was close to the predicted value 1.60% with 0.63% bias.In addition, DPPH· and ABTS· radical scavenging capacity of total flavonoids was better, and IC50 of it was 7.67μg / m L and 12.88μg / m L, respectively.