响应面设计法优化软枣猕猴桃茎中总生物碱的提取工艺

Optimization of total alkaloids extraction from Actinidia arguta stems using response surface methodology

摘要: 以软枣猕猴桃茎为原料,采用乙醇超声波提取法提取软枣猕猴桃茎中总生物碱并测定其含量。以乙醇体积分数、超声波时间、料液比3个单因素为自变量,以软枣猕猴桃总生物碱提取量为响应值,进行响应面实验。用酸性染料比色法测定软枣猕猴桃中总生物碱的含量,优化出软枣猕猴桃茎中总生物碱的最佳提取工艺。结果表明:软枣猕猴桃茎总生物碱的最佳提取工艺为:乙醇体积分数72%、超声时间30min、液料比为43倍。在此条件下,用紫外分光光度法测得软枣猕猴桃茎中总生物碱的提取量为0.542mg/g。

Abstract: The total alkaloids from Actinidia arguta stems were extracted and measured using the method of ultrasonic extraction with ethanol as extraction solvent. Response surface methodology was taken to optimize the extraction of total alkaloids in Actinidia arguta stems.Three elements including ethanol concentration, ultrasonic time and solid- liquid ratio were selected to maximize the yield of total alkaloids. Furthermore, the optimal extraction conditions of total alkaloids from Actinidia arguta stems were 72% ethanol, ultrasonic treatment time 30 min, liquidsolid ratio 43.Under these conditions, the content of total alkaloids was 0.542 mg / g from Actinidia arguta stems by ultraviolet spectrophotometer.