Abstract: Chitosan was chosen as the main film-forming material, added a kind of biological active componentlentinan, with glycerol as the plasticizer, new types of edible composite film were prepared by blade casting process. The influence on composite films of lentinan content and structural characterization tested. Results show that the tensile strength (TS) of films improved from 24.61 MPa to 32.14 MPa with the increasing content of lentinan, while the elongation at break (E) decreased from 15.07% to 4.57%. The water solubility increased as the lentinan increasing, the maximum was 7.04%. The oxygen resistance increased in the initial stage until reaching the best at lentinan content of 1.0%, peroxide value of oil low to 4.28g/100 g, and then flatted. The water vapor permeability decreased in the initial stage until reaching the minimum 0.1322g·mm/ (m2·h·k Pa) at lentinan content of 0.75%, and then increased. IR and XRD analysis showed, that description of composite films had good compatibility between components.