柱前衍生-高效液相色谱法检测食品中的黄曲霉毒素B1、B2、G1、G2

柱前衍生-高效液相色谱法检测食品中的黄曲霉毒素B₁、B₂、G₁、G₂

摘要: 目的:改进柱前衍生-高效液相色谱法测定食品中的黄曲霉毒素B1、B2、G1、G2。方法:分别用甲醇-水（8∶2,v∶v）或二氯甲烷提取食品中的黄曲霉毒素。提取液经免疫亲和柱净化后,采用三氟乙酸（或甲酸）进行衍生,并利用高效液相色谱仪进行测定。结果:黄曲霉毒素B1、B2、G1、G2的检出限分别为0.2、0.2、0.2、0.2μg/kg;在低、中、高加标浓度下的回收率分别为81.0%⁹4.1%、75.6%⁹2.0%、75.0%⁹2.4%、77.6%⁹1.3%。结论:改进后的柱前衍生-高效液相色谱法克服了样品基质的干扰,测定结果更准确。

Abstract: The method was improved to determine aflatoxins B1, B2, G1, G2 in foods by high performance liquid chromatograph with pre-column derivation. Samples were extracted by methanol-water solution (8∶2, v∶v) or dichloromethane according to the different food types. The extracts were cleaned up by immunoaffinity column.After derivation by CF3 COOH or HCOOH, the determination of aflatoxins was performed by high performance liquid chromatography with a fluorescence detector. The detection limits of aflatoxins B1, B2, G1, G2were0.2, 0.2, 0.2 and 0.2μg/kg respectively. The recoveries at different level spiked ranged from 75.0% to 94.1%.The improved method was more accurate for different foods by overcoming the matrix disturbance.