Abstract: Using the mycelium of Flammulina velutipes enriched of germanium as materials, 50 mice were divided into 5 groups randomly: blank group, the blank control group, low dose group, middle dose group, high dose group, then the mice was fed with different dose of the mycelium of F. velutipes enriched of germanium respectively.By detecting the content of blood lactic acid and the content of blood urea nitrogen of mice on the quiet, following exercise and after rest, the effect of the mycelium of F. velutipes enriched of germanium on the ability of anti- fatigue ability of mice was explored. The experimental results showed that each dose experimental group of the mycelium of F. velutipes enriched of germanium could reduce the contents of blood lactic acid and urea nitrogen of mice, and prolong the exhaustion time of loading swimming, and with the increase of the dose of the mycelium, the anti- fatigue ability of mice was enhanced. The mycelium of F. velutipes enriched of germanium had good anti- fatigue ability within the tolerance range of experimental dose.