复合生物保鲜剂结合低温贮藏对扇贝柱7种生物胺含量的影响

韩冬娇; 吴雪丽; 李敬

doi:10.13386/j.issn1002-0306.2015.11.052

复合生物保鲜剂结合低温贮藏对扇贝柱7种生物胺含量的影响

Effect of complex biological preservatives combined with freezing- point storage on the content of seven kinds of biogenic amines

摘要

摘要: 本文以海湾扇贝柱为研究对象,采用柱前衍生高效液相色谱法将酪胺（Tyr）、腐胺（Put）、尸胺（Cad）、组胺（His）、色胺（Try）、精胺（Spd）、亚精胺（Spm）7种生物胺在20min内进行有效分离,并分析了复合生物保鲜剂结合低温贮藏（0、-5、-20℃）过程中扇贝生物胺种类及含量的变化。结果表明,贮藏温度对扇贝中Cad、Spd、Spm的含量变化差异显著（p<0.05）,温度越高,生物胺产生速度越快,Cad、Spm、Spd可作为扇贝贮藏过程中质量变化的参考指标;低温贮藏结合复合生物保鲜剂对扇贝柱中Cad、Spd、Spm的产生有显著的抑制作用。因此,复合生物保鲜剂结合低温贮藏是控制扇贝质量的有效措施。

Abstract: In this paper, pre- column derivatization HPLC was used to seperate seven kinds of biogenic amines including tyramine ( Tyr) , putrescine ( Put) , cadaverine ( Cad) , histamine ( His) , tryptamine ( Try) , spermine ( Spd) and spermidine ( Spm) in bay scallop in 20 min. And the change of biogenic amines amount in scallops was determined and analyzed duiring the storage at 0, - 5 and- 20℃. The results showed that the effect of storage temperature on the content of Spd, Spm and Cad in the scallop was significant ( p < 0.05) , and the higher the temperature was, the faster the three kinds of biogenic amines were produced, so Cad, Spm and Spd can be the reference indexes for the change of quality of scallops during storage.And freezing- point storage combined with complex biological preservatives had significant inhibitory effect on the producing of Cad, Spd and Spm in the scallop column.Therefore, freezing- point storage combined with complex biological preservatives was the effective measure of controlling the quality of scallops.