应用响应面法优化叶黄素降解发酵培养基

Optimization of fermentation medium for lutein degradation applying response surface methodology

摘要: 利用响应面分析法对Pantoea dispersa（Y08）菌降解叶黄素产香的培养基进行了优化。采用Box-Behnken实验设计,选定KH2PO4、蔗糖和混合氮源（酵母膏∶天门冬酰胺=2∶1）3个关键因子为响应因子,以叶黄素降解率为响应值建立多元二次回归方程,在分析各个因素的显著性和交互作用后,确定了Pantoea dispersa菌降解叶黄素的最优培养基为:蔗糖0.97%,混合氮源（酵母膏∶天门冬酰胺=2∶1）1.38%,KH2PO40.15%,叶黄素降解率为80.03%,与理论预测值基本吻合,比优化前提高140.67%。

Abstract: Optimization of fermentation medium using for lutein degradation to form important aroma compounds with Pantoea dispersa Y08 strain was performed employing the response surface analysis ( RSA) method.Based on single factor experiment, three critical factors ( the content of sucrose, mixed nitrogen sources and KH2PO4) were selected as response factors.The quadric regression equation was established according to the lutein degradation yield.As a result, the optimum fermentation medium was composed of sucrose 0.97%, mixed nitrogen sources ( yeast extract∶asparagine = 2∶1) 1.38% and KH2PO40.15%.Under the situation, the highest yield of degradation of lutein was predicted to be 80.03%. After optimization, the lutein degradation yield was increased by 140.67%. The experimental values were in according with the predicted values.