天花粉凝集素对糖尿病大鼠血糖及氧化应激的影响

李琼; 张鹏; 郭晨; 张保顺; 李学刚

doi:10.13386/j.issn1002-0306.2015.10.067

天花粉凝集素对糖尿病大鼠血糖及氧化应激的影响

Effect of Trichosanthes kirilowii lectin on blood glucose and oxidative stress in diabetic rats

摘要

摘要: 目的:研究天花粉凝集素(Trichosanthes kirilowii lectin,TKL)的降糖及其抗氧化作用。方法:糖尿病大鼠分别用生理盐水、盐酸二甲双胍、TKL低、中、高(0.3、0.6、0.9g/kg)剂量灌胃3周,测定大鼠体重、饮食及饮水量,空腹血糖(FBG),糖耐量及大鼠血清中糖化血清蛋白(GSP)、丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)的变化。结果:TKL灌胃3周后,糖尿病大鼠"三多一少"症状明显改善,与模型组对比,高剂量组能明显降低大鼠FBG及血清中GSP、MDA含量,提高大鼠血清中SOD和GSH含量(p<0.01);低、中剂量组可改善大鼠FBG、GSP、MDA和SOD中部分指标含量(p<0.05)。TKL显示出明显改善糖耐量减退的作用。结论:TKL可以明显改善糖尿病大鼠的血糖异常,增强大鼠抗氧化能力。

Abstract: Objective:To expore the hypoglycaemic and antioxidant effects of Trichosanthes Kirilowii lectin (TKL) .Methods:Diabetic rats were orally administrated metformin, saline and TKL low-dose (0.3g/kg) group、middledose (0.6g/kg) group and high-dose (0.9g/kg) for 3 weeks. The body weight, food and water intake, the fasting blood glucose were measured. Oral glucose tolerance tests were carried out to test the severity of diabetic. At the end of the experiment, GSP, MDA, SOD, GSH levels in rats plasma were evaluated. Results : Diabetic symptoms were ameliorated after rats were fed with TKL for 3 weeks compared with diabetic group. High-dose of TKL significantly decreased FBG, GSP, MDA and increased SOD, GSH in diabetic rats (p<0.01) ;FBG, GSP, MDA and SOD (p<0.05) were also improved in the groups of TKL low-dose and middle-dose. Besides, TKL could improve the impaired glucose tolerance in diabetic rats. Conclusion:TKL could effectively decrease blood glucose and improve antioxidant defense of diabetic rats.