Abstract: Objective:To study the anti-ageing effects of polysaccharide from Dendranthema morifolium on aging mice induced by D-galactose and explore the underlying mechanisms. Methods:Healthy female mice were randomly divided into 5 groups:control group, aging model group (80mg/kg·d bw) , and low-, medium- and high-dose polysaccharide groups (50, 100, 150mg/kg·d bw) . The mice were then sacrificed, and the serum, liver and brain tissue were collected. The concentration of malondialdehyde (MDA) in the blood, liver and brain tissue and the activities of superoxide dismutase (SOD) , glutathione peroxidase (GSH-Px) , Catalase (CAT) , total antioxidant activity (T-AOC) in the serum and liver were then measured. The polysaccharide from Dendranthema morifolium had antagonism effects on the mice aging induced by D-galactose, had evident rebound of activities of SOD, GSH-Px, CAT and T-AOC at all three doses in the blood and liver, and had a decrease of MDA in the serum, liver and brain tissue. Conclusion:The polysaccharide from Dendranthema morifolium had anti-aging effects on aging model mice.