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中国精品科技期刊2020
梁洁玉, 朱丹实, 吕佳煜, 葛永红, 曹雪慧, 冯叙桥. 李子采后软化过程中细胞壁酶活性对果胶降解的影响[J]. 华体会体育, 2015, (10): 344-348. DOI: 10.13386/j.issn1002-0306.2015.10.064
引用本文: 梁洁玉, 朱丹实, 吕佳煜, 葛永红, 曹雪慧, 冯叙桥. 李子采后软化过程中细胞壁酶活性对果胶降解的影响[J]. 华体会体育, 2015, (10): 344-348. DOI: 10.13386/j.issn1002-0306.2015.10.064
LIANG Jie-yu, ZHU Dan-shi, LV Jia-yu, GE Yong-hong, CAO Xue-hui, FENG Xu-qiao. Effect of cell wall enzyme activity on the degredation of pectin during postharvest softening of plum fruit[J]. Science and Technology of Food Industry, 2015, (10): 344-348. DOI: 10.13386/j.issn1002-0306.2015.10.064
Citation: LIANG Jie-yu, ZHU Dan-shi, LV Jia-yu, GE Yong-hong, CAO Xue-hui, FENG Xu-qiao. Effect of cell wall enzyme activity on the degredation of pectin during postharvest softening of plum fruit[J]. Science and Technology of Food Industry, 2015, (10): 344-348. DOI: 10.13386/j.issn1002-0306.2015.10.064

李子采后软化过程中细胞壁酶活性对果胶降解的影响

Effect of cell wall enzyme activity on the degredation of pectin during postharvest softening of plum fruit

  • 摘要: 以半边红李子为实验材料,利用质构分析仪研究其常温贮藏软化过程,并考察主要细胞壁酶(果胶酯酶PE、外切多聚半乳糖醛酸酶exo-PG、内切多聚半乳糖醛酸酶endo-PG和葡聚糖苷酶EG)活性变化对果实软化及原果胶降解为可溶性果胶(WSP、ASP、HSP、SSP)的影响。结果表明,李子常温贮藏过程中PE活性变化较小,exo-PG活性一直不断下降,endo-PG活性不断升高,EG活性贮藏前期略有下降,8d之后开始不断升高。李子果肉硬度和粘性降低与原果胶和SSP降解关系密切,ASP降解与粘度降低极显著相关(p<0.01),HSP降解与果肉硬度下降显著相关(p<0.05)。果肉硬度和粘度变化与exo-PG活性极显著(p<0.01)和显著(p<0.05)相关。endo-PG活性变化对果肉硬度下降影响比较明显(p<0.05)。PE活性变化与原果胶、ASP、HSP、WSP含量变化均显著相关(p<0.05);exo-PG活性变化与原果胶、HSP含量变化极显著相关(p<0.01),与SSP、WSP含量变化显著相关(p<0.05);endo-PG对原果胶和HSP的降解影响比较大(p<0.05)。EG对果胶降解影响较小。 

     

    Abstract: To explore the postharvest softening and effect of cell wall enzyme activity (PE, exo-PG, endo-PG and EG) on the degradation of protopectin to souble pectin (WSP、ASP、HSP、SSP) , the experiment was carried out by Banbianhong plum using texture analyzer during storage at ordinary temperature. The results showed that, the changing of PE activity was little, exo-PG activity kept reducing, meanwhile endo-PG activity kept rising during the storage of pulm fruit and EG activity after storing 8d kept rising at ordinary temperature. The changing of pulp hardness and adhesiveness had a close relationship with the content of protopectin and SSP.The content of ASP affected the decrease of adhesiveness significantly (p <0.01) , while HSP affected pulp hardness significantly (p <0.05) . The changing of pulp hardness (p <0.01) and adhesiveness (p <0.05) had significant correlation with exo- PG activity. The changing of endo- PG activity presented a significantly correlation with the decline of pulp hardness (p<0.05) . The activity of PE presented a significantly correlation with the content of protopectin, ASP, HSP and WSP (p<0.05) , exo-PG presented a significantly correlation with the content of protopectin and HSP (p <0.01) , SSP and WSP (p <0.05) . Endo-PG had a great influence on protopectin and HSP (p<0.05) . EG had little effect on the degradation of pectin.

     

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