Abstract: The wound ethylene was produced by fresh-cut, which accelerates the ripening and senescence of fruits and vegetables. The quality change of green-ripe tomato from two different cultivars, wild-type and ethylene receptor Le ETR-3-deficient (Never-ripe, Nr) mutant was investigated during storage at 4 ℃ after fresh-cut processing. Results showed that Nr mutant fruit skin changing to red was retarded, weight loss and the respiration intensity was inhibited, fruit firmness was well maintained, also the decrease of soluble solids, soluble sugar, titratable acid, vitamin C and the chlorophyll 's-degrading of fresh-cut tomato was mitigated compared with the Wt-type, revealing an excellent fresh-keeping effect of Nr mutant tomato during storage.