葵花籽壳水溶性膳食纤维的提取工艺优化及抗氧化活性

Study on the extraction of soluble dietary fiber from sunflower seed shells and its antioxidant activity

摘要: 以葵花籽壳为原料,提取水溶性膳食纤维,研究提取液浓度、料液比、浸提温度及浸提时间对提取率的影响,通过正交实验优化工艺条件,并对其体外抗氧化性进行研究。结果表明,提取最佳工艺条件为料液比1∶30、氢氧化钠的质量分数9.5%、浸提温度40℃、浸提时间30min,葵花籽壳水溶性膳食纤维提取率可达21.32%。葵花籽壳水溶性膳食纤维对1,1-二苯基-2-三硝基苯肼（DPPH）、超氧阴离子自由基（O2-·）和羟自由基（·OH）表现了良好的清除能力,其清除率在样品浓度为1.0mg/m L时分别为86.67%、70.32%和76.33%。

Abstract: The optimization of extraction technology of soluble dietary fiber (SDF) in sunflower seed shells and its anti-oxidative activities were studied. Results showed that the optimized extraction conditions were as follows:solid-liquid ratio 1∶30, extraction solvent 9.5% Na OH, extraction temperature 40℃, extraction time 30 min. Under these conditions, the extraction rate of SDF was 21.32%. The SDF showed strong scavenging activities on1, 1-Diphenyl-2-picrylhydrazyl radical (DPPH) , superoxide radical (O2-·) and hydroxyl radical (·OH) methods, the scavenging rates for the radicals were 86.67%, 70.32% and76.33%, respectively, when the concentration was 1.0mg/m L.