Abstract: Lily flower as the research object, using the response surface analysis methodology the ultrasonic extraction technology of colchicine was optimazed. On the basis of single-factor tests, the effects of ethanol concentration, extraction duration, extraction temperature, and liquid-material ratio on colchicine content were explored. A regression model describing extraction of colchicine as a function of three variables was established with the Design-Expert software according to the Box-Behnken design principle and experimental data. The results showed that the optimal technologies were 60% ethanol as extraction solvent, extraction temperature50℃, material liquid ratio 1∶21 and extraction time 33 min. Under the optimum condition lily alkaloid extracting yield was 16.25μg/g.