响应曲面法优化紫薯中花色苷类物质的提取及抗氧化活性研究

Optimizing the extraction and the antioxidant activity of anthocyanins from purple sweet potato by using response surface methodology

摘要: 以紫薯为原料,利用热水提取紫薯花色苷,以提取物中紫薯花色苷的含量和抗氧化活性作为衡量提取工艺的指标,并利用响应曲面法进行实验设计及工艺参数优化。结果表明:紫薯花色苷的最佳提取条件为柠檬酸质量分数3%,提取温度63℃,提取时间2h,液料比为40∶1m L/g。在此条件下紫薯花色苷的含量可达7.21mg/g,同时抗氧化活性(IC50值)为123.93μg/m L。该提取方法准确、可靠,可用于紫薯中花色苷类物质的提取,同时提取物具有最强的抗氧化活性。

Abstract: The response surface methodology was used to optimize the hot water extraction of anthocyanins from purple sweet potato. Three parameters including citric acid mass fraction, extraction temperature and extraction time were optimized based on anthocyanins content and the antioxidant activity of the extract. The optimum extraction conditions were citric acid mass fraction 3%, extraction temperature 63℃ and extraction time 2h for anthocyanins. Under this extraction condition, the extraction rate of purple sweet potato anthocyanins was 7.21mg/g, and the IC50 value for DPPH radical scavenging activity was 123.93μg/m L. The results suggested that the regression models were accurate and adequate for anthocyanins extraction from purple sweet potato, while the extraction showed the highest antioxidant activity.