山杏仁种皮多酚的闪式提取工艺优化及体外抗氧化活性研究

Extraction technology and antioxidant activity analysis of polyphenols from apricot (Armeniaca sibirica) seed coat

摘要: 利用闪式提取法对山杏仁种皮中多酚的提取工艺进行优化,并通过DPPH法、邻苯三酚法、水杨酸法以及还原能力实验,研究了其体外抗氧化能力。得到最佳工艺条件为:料液比1∶20（g/m L）、乙醇体积分数30%、闪提时间120s,多酚得率为9.650mg/g。结果表明:当多酚提取物浓度为0.4mg/m L时,对DPPH·的清除率为92.9%;浓度为3.0mg/m L时,对O2-·清除率为93.1%;浓度为2.0mg/m L时,对OH·的清除率为98.1%;浓度为1.0mg/m L时,还原力为1.411。

Abstract: The optimum extraction condition of polyphenols from apricot seed coat with the herbal blitzkrieg extractor and the antioxidant activity were investigated in this paper. The four antioxidant assays were contained total reductive ability, scavenging ability for DPPH·, ·OH, and O2-·. The optimum extraction condition was listed:solid-liquid ratio 1∶20 (g/m L) , ethanol concentration 30% and time 120 s. Under this situation, the extraction rate of polyphenols was 9.650mg/g. When the polyphenols concentration was 0.4mg/m L, the rate of scavenging DPPH· was 92.9%. When it was 3.0mg/m L, the rate of scavenging O2-· was 93.1%. When it was 2.0mg/m L, the rate of scavenging OH· was 98.1%. When it was 1.0mg/m L, reducing force was 1.411.