响应面法优化凤丹籽饼粕中单萜苷的超声提取工艺

Response surface optimization of ultrasonic-assisted extraction of total monoterpene glycosides from seed cake of Paeonias ostii

摘要: 为确定凤丹籽饼粕中单萜苷的最佳超声提取工艺条件,研究了超声时间、乙醇体积分数和液料比3个主要因素对单萜苷提取率的影响。在单因素实验基础上,采用响应面法对超声提取凤丹籽饼粕中单萜苷的工艺参数进行优化。得到最佳工艺条件为:超声时间35min,乙醇体积分数为41%,液料比为35∶1(m L/g),在此工艺条件下的单萜苷的得率为7.089%。

Abstract: To investigate the optimal extraction conditions of monoterpene glycosides from seed cake of Paeonia ostii under ultrasonic assistance, three extraction parameters including ultrasonic time, ethanol concentration and liquid/material ratio on the extraction rate of monoterpene glycosides were optimized. On the basis of single factor tests, the extraction technology of monoterpene glycosides from the seed cake of Paeonia ostii was optimized by response surface methodology. The optimum conditions obtained were as follows:extraction time was 35 min, ethanol concentration was 41%, liquid/material ratio was 35∶1 (m L/g) . Under this conditions, the extraction rate values of monoterpene glycosides was 7.089%.