新疆野生苹果(Malus sieversii)的总多酚、总黄酮提取物的抗氧化活性研究
Antioxidant activity of polyphenolics and flavonoid extracted from wild apple (Malus sieversii)
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摘要: 以采集于新疆不同地区的20个野生苹果品种为研究对象,福林酚法测总多酚,分光光度计法测定总黄酮,DPPH、ABTS、还原能力法测定总抗氧化能力,相关系数法分析各个指标之间的相关性。结果显示,不同品种的野生苹果的总多酚、总黄酮及抗氧化活性之间具有显著差异,其中,HDM-23的总多酚含量最高(4714.33μmol没食子酸/100g鲜果),XY-31的总黄酮含量最高(2452.84mg芦丁/100g鲜果)。总多酚、总黄酮及抗氧化活性之间呈极显著(p<0.01)正相关,表明总多酚与总黄酮是野生苹果抗氧化活性的主要贡献物质。Abstract: Twenty different lines of wild apple (Malus sieversii) collected from different places in Xinjiang, China were used as research objects. Total total polyphenol content was measured using the Folin- Ciocalteau method. Total flavonoid content was measured by spectrophotometer. Total antioxidant capacities were analyzed by ABTS assay, DPPH assay, and iron ( Ⅲ) to iron ( Ⅱ) reducing power assay, and the correlation between the various indicators was measured with Pearson correlation coefficient. It was found that HDM-23 had the highest total polyphenol content (4714.33μmol gallic acid/100 g fresh matter) , and XY-31 contained the highest amount of total flavonoid content (2452.84 mg rutin/100 g fresh matter) . The present study implied that total polyphenols contribute to the antioxidant properties of the wild apple (p<0.01) and total flavonoid was an important phenolic compound in the wild apple varieties investigated.