Abstract: The rapid detection method of triazophos residues in flesh of navel orange was reported in this paper.Flesh of navel orange was obtained using quick pre-treatment method. SERS of different concentration of triazophos solutions with flesh extract as the matrix were collected by laser Raman spectrometer. The results showed that minimum detection concentration for triazophos in navel orange was 0.5mg/L. Acetonitrile was selected as the internal standard and the relative intensity was made by characteristic peak ( 1409cm-1) of triazophos and characteristic peak (2257cm-1) of acetonitrile. A good linear relationship was found between the relative intensity and concentration of triazophos in the range of 0.5²0mg/L, with a good correlation coefficient (R2) of 0.9919. The predict recoveries were 98.1%¹02.5% and the predicted deviations were below 0.12mg/L.This study demonstrates that this method was accurate and reliable.