柱前衍生化高效液相色谱法分析发菜胞外多糖的单糖组成

Analysis of monosaccharide compositions of Nostoc Flagelliforme extracellular polysaccharide by pre-column derivation HPLC

摘要: 采用水溶醇沉法提取发菜多糖,通过三氟乙酸（TFA）将其水解成单糖,经1-苯基-3-甲基-5-吡唑啉酮（PMP）衍生化后,利用RP-HPLC分析其单糖组成。结果表明,发菜胞外多糖由甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖、半乳糖、木糖组成,其摩尔比为0.71∶0.37∶0.44∶1.53∶1.03∶0.40。该方法具有操作简便、快捷,重现性良好等特点,可用于发菜胞外多糖中单糖组成的定性和定量分析。

Abstract: The polysaccharide was precipitated by ethanol, and hydrolyzed into monosaccharides by trifluoroacetic (TFA) . The hydrolyzate was derivated with 1-phenyl-methyl-5-pyrazolone (PMP) and then the PMP derivates of monosaccharides were determined by RP-HPLC. The results showed that Nostoc Flagelliforme extracellular polysaccharide was composed of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and the molar ratio of the six monosaccharides were 0.71∶0.37∶0.44∶1.53∶1.03∶0.40. The established HPLC method was simple, fast and good reproducibility, and could be used for the determination of monosaccharide compositions of Nostoc Flagelliforme extracellular polysaccharide.