苦丁茶叶制虫茶粗多酚对HepG2人肝癌细胞的凋亡诱导效果

周雅琳; 冯霞; 朱凯; 赵欣

doi:10.13386/j.issn1002-0306.2015.09.065

苦丁茶叶制虫茶粗多酚对HepG2人肝癌细胞的凋亡诱导效果

Apoptosis inducing effects of crude polyphenols of Insect tea made by Kuding tea leaves in Hep G2 human hepatoma cells

摘要

摘要: 茶多酚是茶叶中的功能性成分。虫茶作为传统饮品也含有这些化合物,这些多酚类物质的抗癌效果有待科学的研究。本研究对苦丁茶叶制虫茶粗多酚（CPKMI）的癌细胞凋亡诱导作用进行了测定。采用MTT法对CPKMI对癌细胞的体外生长抑制作用进行了分析,然后进一步采用RT-PCR检测对CPKMI的癌细胞凋亡诱导效果进行了实验。经过25、50和100μg/m L的CPKMI处理癌细胞48h,Hep G2人肝癌细胞的增殖被抑制,其中100μg/m L的CPKMI表现出最高的抑制率（72.8%）。CPKMI也可以通过上调caspase-3、caspase-9、p53、p21、E2F1、p73和下调HIAP-1,HIAP-2基因的mRNA的表达对Hep G2癌细胞起到显著的凋亡诱导效果（p<0.05）。由此可见,CPKMI表现出强的体外癌细胞凋亡诱导效果。

Abstract: Tea polyphenols are functional substances present in tea. Insect tea as a traditional drink also contains these compounds,the anticancer effects of these polyphenols need to be scientific researched. In this study,cancer cells apoptosis inducing effects of crude polyphenols of Insect tea made by Kuding tea leaves( CPKMI)were determined.The in vitro cancer cells growth inhibitory effects of CPKMI were checked by MTT assay,the apoptosis inducing effects of CPKMI were further tested by RT- PCR assay. After 25,50 and 100μg / m L of CPKMI treatment for 48 h,cell proliferation of Hep G2 human liver cancer cells were inhibited,and the 100μg / m L of CPKMI showed the highest inhibitory rate at 72.8%.CPKMI also significantly induced apoptosis in Hep G2 cancer cells( p <0.05) by upregulating caspase- 3,caspase- 9,p53,p21,E2F1,p73 and down regulating HIAP- 1,HIAP- 2 mRNA expressions.CPKMI thus present strong cancer cells apoptosis inducing effects in vitro.