Abstract: Using Ginkgo callus as material,the optimization of the extraction conditions of Ginkgo flavonoids was done.Through single- factor tests,the influences of several factors on extraction rate of Ginkgo flavonoids were investigated,such as ethanol concentration,liquid- powder ratio,microwave power,microwave processing time,water bath temperature,water bath processing time,etc. Then,orthogonal test was carried out. The results of orthogonal experiment indicated that: using 40% ethanol as the extraction solvent,liquid- powder ratio 25 ∶ 1,microwave processing 60 s under middle power level( 528W),no water bath treatment,the extraction rate of flavonoids reached 20.92 mg / g.