Abstract: Objective: To prevent the microbial contamination of the process of broiler slaughtering. Methods:Investigation of total bacterial count of carcass in workshop environment during segmentation and the number of colonies,Eseudomonas,Coliforms and Enterococcus after segmentation of products and detection of the situation of Staphylococcus aureus,Salmonella of them in the study. And the orthogonal experimental design was investigated to optimize the conditions of the drenching. Results: During the segmentation process,the number of bacteria in the air and process water did not exceed the national standard,but the contact surface pollution was more serious,mostly in unacceptable levels. After segmentation the number of colonies,Pseudomonas,counts of Coliforms and Enterococcus were 3.85 ~ 4.17,3.14 ~ 3.87,2.86 ~ 3.68,1.07 ~ 1.82lg( cfu / cm2) on chicken products surfaces.The detection rates of Staphylococcus aureus and Salmonella were respectively 46.67%,14.17%. The suitable conditions for the reduced colonies were 0.5%( w / v) lactic acid,1.0%( w / v) citric acid and drenching time30 s,it could reduce the total number of colonies 1.97lg( cfu / cm2).Compared with the control group,the group of minus bacteria had no effect on the surface of the chicken color,odor and taste of heated broth. The final total number of colonies in chicken products was 2.20lg( cfu / cm2). Conclusion: The split environment of broiler carcasses and bacterial contamination of split chicken products are more serious,and there is pathogen contamination. Lactic acid combined with citric acid can significantly reduce the total number of colonies of chicken's surface after splitting.