诱导物及种间诱导对白腐菌的锰过氧化物酶(MnP)的诱导

钟紫旋; 刘丽; 张勇; 罗锋

doi:10.13386/j.issn1002-0306.2015.09.028

诱导物及种间诱导对白腐菌的锰过氧化物酶(MnP)的诱导

Induction on the Manganese Peroxidase of white-rot fungi by exogenous inducers and interspecific induction

摘要

摘要: 本实验通过添加外源诱导物诱导和不同白腐菌种间相互诱导两种途径来研究Trametes versicolor、Pleurotus ostreatus、Dichomitus squalens、Bjerkandera adusta四种白腐菌产Mn P活性,发现这些白腐菌在一些诱导条件下可产生大量Mn P。结果表明,外源诱导物中,2,5-二甲基苯胺是有效的Mn P诱导剂,对四种菌产Mn P均有不同程度的诱导作用。异丙醇能提高T.versicolor、P.ostreatus和D.squalens产Mn P活性,且诱导效果优于乙醇。Cu2+能显著提高T.versicolor和D.squalens产Mn P活性。Mn2+能显著提高P.ostreatus和B.adusta产Mn P活性。不同白腐菌相互作用时,T.versicolor、P.ostreatus和D.squalens三种菌两两共培养均有明显的Mn P种间诱导效果。本研究为大量产Mn P并将其应用于工业提供可能。

Abstract: The study investigated the Mn P activity of Trametes versicolor,Pleurotus ostreatus,Dichomitus squalens,Bjerkandera adusta by two kinds of induction,exogenous inducer and interspecific induction between different fungi,and discovered that in some induction condition,these white- rot fungi could produce large amounts of Mn P.The results showed that in exogenous inducers,2,5- dimethylaniline was an effective Mn P inducer,which could enhance Mn P activity of four fungi to different extent.Isopropyl alcohol could enhance Mn P activity of T.versicolor,P.ostreatus and D.squalens,and it was better than that of ethanol.Cu2 +could significantly enhance Mn P activity of T.versicolor and D. squalens. Mn2 +could significantly enhance Mn P activity of P. ostreatus and B. adusta. When induced by interspecific induction between different fungi by co- cultivation,T. versicolor,P. ostreatus and D.squalenss could strongly enhance Mn P activity each other. The study provided possibility for large production of Mn P and being applied to industry.