Abstract: The ability of Lactobacillus plantarum B7 and L.pentosus X8 to bind fumonisins was studied.The removal of fumonisins,FB1 and FB2 by strains B7 and X8 was detected by reversed- phase HPLC with fluorescence detector.The percentage of FB1- binding was 52.9% for strain B7 and 58.0% for strain X8 respectively. Results showed that more FB2 was bond to two strains when incubated at 37℃ for 1h.The removal percentage of FB2 by strains B7 and X8 was 85.2% and 86.5%,respectively.Factors which affect the ability of the two strains to remove FB1 and FB2 included temperature,p H and enzyme.The fumonisins- binding does not depend on the activity of the two lactobacilli strains.The higher p H values,the less the removal percentage of fumonisins by the two strains. No fumonisins- binding by strain B7 and X8 was detected when p H was larger than 7. The cells of two lactobacilli strains subjected to the pretreatment of boiled water for 15 min showed higher removal of fumonisins than their controls.The lysozyme was used to treat the cells of two lactobacilli strains significantly reduced their ability to bind fumonisins.The selected two lactobacilli strains should be potential candidates as a biological detoxification agent for the remove of fumonisins via their physical binding,although more FB2 was removed than FB1.