Abstract: The contents of phenolic compounds from Lentinus edodes( Berk.) sing.under four drying methods were determined by Folin- Ciocalteu method. The superoxide anion radicals,hydroxyl radicals and DPPH radicals scavenging capacities of phenolic compounds from Lentinus edodes( Berk.) sing.were determined and compared.It was found that drying method had significant effect on the content of phenolic compounds from Lentinus edodes( Berk.) sing.( p < 0.05). The contents of phenolic compounds in Lentinus edodes( Berk.) sing. dried by freeze drying,hot- air drying,vacuum drying and air drying were 32.35 ± 0.24,23.21 ± 0.12,22.80 ± 0.25,( 14.72 ± 0.11) mg GAE / g,respectively. Phenolic compounds of Lentinus edodes( Berk.) sing. had certain scavenging capacity for superoxide anion radicals,hydroxyl radicals and DPPH radicals.In 0~10.00 mg / m L concentration range,scavenging capacity was correlated with concentration. Antioxidant activities of phenolic compounds from Lentinus edodes( Berk.) sing. processed by different drying method were significantly different. The three radicals scavenging capacities of total phenolic compounds followed the decreasing order: freeze drying,hot- air drying,vacuum drying and air drying.