枣花蜜和荞麦蜜中葡萄糖氧化酶的活性及热稳定性研究
Research of activity and thermal stability of GOD in jujube and buckwheat honeys
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摘要: 分别测定了不同地理源的枣花蜜和荞麦蜜中葡萄糖氧化酶(glucose oxidase,GOD)的活性。在测定的枣花蜜样品中陕西佳县枣花蜜GOD活性最高,为(0.161±0.002)U/g,山西吕梁枣花蜜GOD活性最低,为(0.007±0.001)U/g;荞麦蜜中四川西昌荞麦蜜GOD活性最高,为(0.112±0.001)U/g,山西晋北荞麦蜜GOD活性最低,为(0.007±0.001)U/g。结果表明,蜂蜜植物源不同,GOD活性不同;植物源相同时,地理源不同,蜂蜜中GOD活性差异也较大。对GOD的热稳定性研究表明,枣花蜜中的GOD在70℃加热90min活性完全损失,荞麦蜜GOD活性仍有部分残存,表明四川西昌荞麦蜜中的GOD热稳定性较陕西佳县枣花蜜高。Abstract: The activities of glucose oxidase in jujube and buckwheat honey of different geographical sources were determined.Jujube honey from Jiaxian,Shanxi Province and buckwheat honey from Xichang,Sichuan Province had the highest glucose oxidase activity with the value of( 0.161 ± 0.002) U / g and( 0.112 ± 0.001) U / g,respectively. The activity of GOD in Lvliang,Shanxi jujube honey samples was only( 0.007 ± 0.001) U / g,and the lowest activity appeared in buckwheat honey from Jinbei,Shanxi Province with the same value.The results showed that the GOD activity varied significantly between different botanical and geographical sources of Chinese unifloral honey.Furthermore,thermal stability of GOD in these two honey samples was detected. The result showed that with increasing of temperature,the activity of GOD in jujube was reduced and there was no activity when the temperature up to 70℃ with 90 min. Under the same conditions,the activity of GOD still can be determined in buckwheat honey.This research indicated that the thermal stability of buckwheat honey was higher than jujube honey.
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