HPLC法同时检测复方产品中西洋参、红景天和刺五加的皂苷含量

郭栋; 张振华; 于淼; 赵升强; 王楚绮; 张娟; 赵海锋

doi:10.13386/j.issn1002-0306.2015.07.049

HPLC法同时检测复方产品中西洋参、红景天和刺五加的皂苷含量

Simultaneous determination of saponins in the mixture extracts of Panax quinquefolius,Rhodiola rosea and Eleutherococcus senticosus by HPLC

摘要

摘要: 采用高效液相色谱法建立了同时检测复方产品中人参皂苷Rb1、人参皂苷Re、红景天苷、刺五加苷B和刺五加苷E含量的方法。以60%乙醇作为提取溶剂,用色谱柱Kromasil 100-5C18（250×4.6mm,5μm）进行分离,以0.1%磷酸-乙腈混合液为流动相进行梯度洗脱,结果表明,在此条件下5种皂苷得到了良好的分离。人参皂苷Rb1、人参皂苷Re、红景天苷、刺五加苷B和刺五加苷E分别在80⁸00、80⁸00、40⁴00、20¹60μg/m L和40³20μg/m L范围内呈现良好的线性关系,平均加标回收率在99.5%¹06.8%之间,相对标准偏差为0.5%².3%。本方法具有重现性好、回收率高等优点,适用于复方产品中人参皂苷Rb1、人参皂苷Re、红景天苷、刺五加苷B和刺五加苷E的同时检测。

Abstract: HPLC has been applied to determine the contents of ginsenoside Rb1,ginsenoside Re,salidroside,acanthopanax B and acanthopanax E in the mixture extracts of Panax quinquefolius,Rhodiola rosea,and Eleutherococcus senticosus.The samples extracted with 60% alcohol were separated on a Kromasil 100-5 C18column( 250 × 4.6mm,5μm),and were eluted with 0.1% phosphoric acid-acetonitrile at a flow rate of 0.9m L/min under gradient elution conditions. The results showed that all of the saponins in the mixture extracts were well separated on HPLC. The linear ranges of the ginsenoside Rb1 and ginsenoside Re,salidroside,acanthopanax B and acanthopanax E were 80 ~ 800,80 ~ 800,40 ~ 400,20 ~ 160μg/m L and 40 ~ 320μg/m L,respectively. Their average recovery rates were 99.5% ~ 106.8%,with the relative standard deviations of 0.5% ~ 2.3%. In conclusion,the proposed method had a good linearity and sensitivity which was suitable to detect simultaneously the contents of the ginsenoside Rb1,ginsenoside Re,salidroside,acanthopanax B and acanthopanax E in the mixture extracts or formulations.