小球藻保健饮料的研制
Study on the health drink of Chlorella
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摘要: 以小球藻粉为原料,采用超声波辅助提取藻多糖,再利用木瓜蛋白酶与中性蛋白酶进行水解藻蛋白,合并滤液后,按一定比例添加适量蜂蜜、白糖、柠檬酸直接配制澄清型小球藻保健饮料。通过正交实验确定提取藻多糖的最佳工艺为:料液比1∶15(g∶m L),p H7,温度60℃,时间30min,小球藻多糖提取得率为18.94mg/g。水解藻蛋白的最佳工艺为:底物浓度100g/L,混合酶量12000U/g,p H6.5,温度55℃,小球藻蛋白水解度达27.83%。研制成的产品营养丰富、风味独特,是一款老少皆宜的营养保健饮品。Abstract: With Chlorella powder as raw material,the algae polysaccharide was extracted by the method of ultrasonic assisted,then papain and neutral protease were used to hydrolyze alga protein. A kind of health drink was manufactured by adding right amount honey,sugar and citric acid into the filtrate. The optimal extraction condition by orthogonal test was as follows: solid-liquid ratio of 1 ∶ 15( g ∶ m L),p H7.0,extraction temperature of60℃,extraction time 30 min,under which condition,extraction yield of Chlorella polysaccharide was 18.94 mg/g.The optimal condition of hydrolyzing alga protein was as follows: substrate concentration of 100 g/L,the amount of composite enzyme of 12000 U/g,p H6.5,temperature of 55℃. Under which the degree of hydrolysis of Chlorella protein was up to 27.83%.The nutritional health drink was obtained with rich nutrition,unique flavor.