Abstract: Objective: To understand the relationship between drug-resistant gene and drug resistance of Salmonella isolates from broiler slaughterhouse production chain. Method: The susceptibility testing of 72 Salmonella strains from broiler slaughterhouse production chain to 10 varieties of antibiotics were performed by means of K-B disc method and PCR technique was used to detect the presence of resistance gene in the corresponding drug-resistant Salmonella isolated. Result: The nalidixic acid( 100%) resistant rates of Salmonella isolated from broiler slaughterhouse production chain were the highest.The resistant rates of 72 Salmonella isolated to ampicilin,benzyl methyl oxygen/sulfamethoxzole,ciprofloxacin,tetracycline,gentamicin were 69.44%,54.17%,38.89%,34.72% and13.89%,respectively.At least one antimicrobial resistance gene was detected in each Salmonella isolate and the following antimicrobial resistance genes were commonly present; the detection rates of the resistant strains of bla TEM,bla CMY-2,sul2,sul3,tet B and tet C genes were 96.08%,100%,94.87%,71.79%,78.38%,100%,respectively.Conclusion: These results implied that the resistant Salmonella isolated from broiler slaughterhouse production chain to common antibiotics were different from each other,and resistant genes were widely existed in these resistant strains. Antimicrobial phenotypes of Salmonella isolates were very consistent with their genotypes( ≥75%).