Abstract: In this paper,using Corynebacterium crenatum mutagenesis as tester strain,the effects of medium components( carbon,organic nitrogen,inorganic nitrogen,inrotrogen ions) and the fermentation conditions( incubation temperature,inoculum size,initial p H,the volume of fermentation broth) on arginine production were investigated through shake flask. The results indicated that the optimal L-arginine-producing medium was composed of 12% Glucose,4.5% ammonium sulfate,2.5% corn steep liquor,0.005% potassium dihydrogen phosphate,0.01% seven water magnesium sulfate,0.01% six water ferric chloride,0.05% Manganese sulfate monohydrate and 3% calcium carbonate. The optimal fermentation conditions were 30℃ of culture temperature,10% of inoculum concentration,7.0 of initial p H and 15 m L /250 m L of loading volume. On the base of the optimal fermentation medium and fermentation conditions,the production of arginine increased by 27.7% and reached11.23 g/L.