响应面法优化姜黄素的提取工艺及其抗氧化活性的研究

Study on optimization of extraction process for curcuminoids by response surface method and its antioxidant activity

摘要: 采用响应面法优化了姜黄素的提取工艺,并探讨了姜黄素对超氧阴离子自由基（O2-·）、羟基自由基（·OH）、DPPH自由基、ABTS+自由基的体外抗氧化活性。姜黄素的最佳提取工艺条件为乙醇浓度85%,超声时间50min,液料比20∶1（m L/g）。在此条件下,姜黄素得率为1.538%。体外抗氧化实验表明,三种姜黄素标准品具有较强的抗氧化活性且各有差异,姜黄素提取液对超氧阴离子自由基（O2-·）、羟基自由基（·OH）、DPPH自由基、ABTS+自由基也具有较强的清除能力。

Abstract: Response surface methodology was used to optimize the extraction process for curcuminoids. The antioxidant activity of curcuminoids was evaluated using superoxide anion(O2-·),hydroxyl(·OH),DPPH and ABTS+radical scavenging. The results indicated that the optimal extraction conditions were ethanol concentration(V/V) 85%,ultrasonic time 50 min,and material-to-liquid ratio 20∶1(m L/g). Under these extraction conditions,maximum yield of curcuminoids of 1.538% was obtained. The antioxidant experiment in vitro demonstated that the antioxidant activity of three curcumin standards were strong and different. Meanwhile the curcuminoids extract also have a better ability to scavenge superoxide anion(O2-·),hydroxyl(·OH),DPPH and ABTS+radical.