黑米花青素体内抗氧化研究

石娟; 张曼莉; 孙汉巨; 陈晓燕; 娄秋燕; 刘宁; 王霄

doi:10.13386/j.issn1002-0306.2015.05.065

黑米花青素体内抗氧化研究

Study on in vivo antioxidation of anthocyanins from black rice

摘要

摘要: 本文研究了黑米花青素对小白鼠的抗氧化及免疫调节作用。以生理盐水为载体,维生素C作为阳性对照,分别采用高、中、低三种剂量的黑米花青素粗提物及其纯化物,灌胃喂养小白鼠30d后,测定小白鼠的脾和胸腺指数,血清、肝脏、心脏和肾脏中总抗氧化力（T-AOC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化酶（GSH-Px）的活力,丙二醛（MDA）和羟自由基含量。研究结果表明:该花青素能增加小白鼠的脾和胸腺指数,显著提高其体内T-AOC水平和SOD、GSH-Px的活性,降低MDA和羟自由基的含量。因此,黑米花青素具有提高小白鼠的体内抗氧化及免疫调节作用。

Abstract: This paper studied the in vivo antioxidation and immune modulation functions of anthocyanins from black rice.Saline solution was used as carriers,while vitamin C was used as positive control. Mice were fed with crude and pure anthocyanins at high,medium and low doses,respectively. After feeding for 30 days by mean of irrigation,the spleen and thymus indexes were detected,while T- AOC,SOD,GSH- Px,MDA and hydroxyl radical contents of serum,liver,heart and kidney were also determined.The results indicated that the anthocyanins could increase the spleen and thymus indexes,significantly increase the level of T- AOC,the activity of SOD and GSHPX,and reduce the contents of MDA and hydroxyl radical.Therefore,the anthocyanins have the functions of in vivo antioxidation and immune modulation.