Abstract: Considering of the cellulosic biofuel,there is an increasing number of commercial cellulase preparations on market.Because of the different substrate dependent of commercial cellulases,their hydrolytic performances are totally different.In order to explore the hydrolytic behavior of commercial cellulase,the features of Cellic CTec2( Ctec 2) on hydrolyzing atmospheric glycerol pretreated wheat straw were studied by measuring hydrolysis efficiency.The results showed that hydrolysis efficiency of CTect2 was higher than the other three commercial cellulase preparations- Celluclast 1.5 L,GC220 and Spezyme- CP. Also there was no synergy occurring between them.At a high consistency of the substrate,CTect2 showed an extraordinary hydrolytic rate,reaching 39.1% for72 h at 15% substrate concentration with a loading of 8mg protein / g glucan. However,the other three cellulases almost did not hydrolyze the substrate at same condition.Additionally,some additives were also helpful for CTect 2in hydrolysis of atmospheric glycerol pretreated substrate.The supplement of 0.05 g / g PEG10000,tween20 and BSA resulted in an increase of hydrolysis rate of 55.3%,42.5% and 38.3%,respectively.In conclusion,Ctec2 had some outstanding advantages,especially in high consistency hydrolysis of lignocellulose.