Abstract: Anti-fatigue peptides was a kind of bioactive peptides,which had an effect of relieving fatigue and increasing human energy. In recent years,with the development of functions of bioactive peptides and the increase in social demand,the research of anti-fatigue peptides received widespread attention. The current literature,at home and abroad,relating to anti-fatigue mechanism,preparation methods,determination methods,the research status were summarized and its direction of future development and problems existed were simply introduced. The aim was to provide some references for further research on anti-fatigue peptides.