Abstract: Seeds of Cabernet Sauvignon were crushed under the different conditions of temperature and time to find out the effect on broken grape seed cell rate. Using the method of calculation of ideal crush model for reference,broken grape seed cell rate was calculated by measuring the diagram of grape seed cell,particle size of grape seed superfine powder,and probability distribution of particle size. Our data demonstrate broken grape seed cell rate rised obviously during the extending of crush time at the same crush temperature,with a linear relation under-15℃ and-25℃. While the rate rised slowly with the decrease of crush temperature in the same crush time. When the crush time extended to 75 min,the broken cell rate was high and influence of temperature was low. The broken cell rate of the superfine powder whose particle size was smaller than the diagram of grape seed cell was 100%,while the broken cell rate of whose particle size was bigger than the diagram of grape seed cell diminished with the increase of particle size. The better crush condition recommended was-15℃ with 75 min.