分段提取香茅草精油及其对食用油脂的抗氧化活性研究
Study on antioxidant activity of lemongrass essential oil by stage extraction
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摘要: 目的:提取云南产香茅草精油,研究不同提馏段精油对食用油脂的抗氧化活性。方法:采用水蒸气蒸馏法分段提取香茅精油,并以猪油和菜籽油为介质,过氧化值(POV)为指标,考察香茅精油的抗氧化活性,以BHT和BHA做对照。结果:水蒸气蒸馏法提取香茅精油的最佳工艺为:料液比为1∶20,Na Cl的质量浓度为7g/L,提取时间为160min,总出油率为1.23%。GC-MS分析前20min和20160min香茅精油中柠檬醛、橙花醛、橙花醇等主要成分的含量差别较大。香茅精油对猪油的抗氧化活性较低。20160min香茅精油对菜籽油有较好的抗氧化活性,与VC复合使用时,其对菜籽油的抗氧化活性与BHT相当。20160min精油对DPPH·的清除能力较BHT和BHA差,而其总抗氧化活性与BHT和BHA相当。结论:香茅草精油具有较好的抗氧化活性,并且精油中高沸点物质的活性大于低沸点物质的活性。Abstract: Objective:To get Yunnan Lemongrass essential oil by stage extraction,the paper investigates the antioxidant activity on edible fats. Methods :With steam distillation stage extraction to obtain the essential oil.With the lard and rapeseed oil as the medium,and the Peroxide Value as the index,the antioxidant activities of the tracts and its collaborative antioxidant effect with VCwere investigated. The various antioxidant activities were compared with the BHT and BHA. Results:The technology conditions for extracting the essential oil from the lemon grass by steam distillation were as follows:the material-liquid ratio of 1 ∶20,Distillation time of 160 min and the mass concentration of Na Cl was 7g/L,the total oil yield was 1.23%. The extraction process of volatile oils was divided into two stages considering the color change of the oils. The distillation time was first20 min and 20~160min. The contents of the main components of the oils were obviously different in two fractions.The 20 ~160min fractions had good antioxidant activity to rapeseed oil and had same antioxidant activity with BHT when used with VC. But antioxidant activity of lemongrass essential oil in lard was low. The DPPH·scavenging ability of 20~160min fractions was worse than BHT and BHA,but had same total antioxidant activity with them. Conclusion:Lemongrass oil had good antioxidant activity,and compounds of high boiling point demonstrated more antioxidant activity than low ones.