产CLA乳酸菌体内、体外抑菌活性的研究

Study on antibacterial activity of conjugated linoleic acid producing Lactobacillus in vivo and vitro

摘要: 采用体内和体外抑菌法研究产CLA乳酸菌对大肠杆菌、金黄色葡萄球菌和沙门氏菌的抑菌作用。体外抑菌实验表明,乳酸菌发酵上清液对大肠杆菌的抑制作用最强,对沙门氏菌和金黄色葡萄球菌的抑制作用相对较弱。体外研究还发现,乳酸菌发酵液在pH<5.0时,抑菌效果较好,并且具一定的热稳定性,对过氧化氢酶和蛋白酶的抗性较高。体内抑菌实验表明,该乳酸菌能够有效降低以上三种致病菌引起的小鼠死亡率,且体内抑菌效果随剂量的增加而增强。结论:产CLA乳酸菌在体内和体外均有较好的抑菌效果。

Abstract: To evaluate and compare the antibacterial activity of conjugated linoleic acid producing Lactobacillus(CLA-producing Lactobacillus) to E.coli,Staphylococcus aureus and Salmonella in vivo and vitro. In vitro test results showed : the antibacterial activity of LAB against E. coli was better than Staphylococcus aureus and Salmonella. The Lactobacillus showed high temperature stability up to 90℃,stability in the rage of pH2.0~5.0and anti-protease activity and anti-catalyase activity. In vivo,the Lactobacillus had more powerful bacteriostatic effect and enhanced bacteriostatic effect to be with increasing does. Conclusions : The CLA- producing Lactobacillus was proved to be of bacteriostatic actions in vitro and in vivo.