Abstract: Objective:Two methods for determining entrapment efficiency of juglone liposome were established by comparing the determination results of two method. Methods:Dialysis and dextran gel methods were applied to separate free drugs from liposomes followed by measuring encapsulated Juglone on a UV spectrophotometer.Results :14h was needed for dialysis method to reach dialysis equilibrium. Free drugs could be effectively separated in 30 min by dextran gel method. The content of Juglone showed good linear relationship in the concentration range of 0. 005 ~ 0. 04 mg / m L at the wavelength of 425 nm. Through the recovery experiment,precision and reproducibility experiment confirmed that the two methods were feasible. Conclusion : A comparision of the two methods suggested that the dialysis method was time-consuming and the resulting value was low,the dextran gel method required less time,was more convenient and the entrapment efficiency measured was closer to the true value.