以线虫为模型评价唾液乳杆菌延长寿命及对相关生理活动的影响

范金波; 侯宇; 蔡茜彤; 周素珍; 冯叙桥

doi:10.13386/j.issn1002-0306.2015.04.020

以线虫为模型评价唾液乳杆菌延长寿命及对相关生理活动的影响

Extending life span effects of Lactobacillus saliva in Caenorhabditis elegans

摘要

摘要: 利用秀丽隐杆线虫,评价了三株唾液乳杆菌对线虫寿命及生理功能的影响。结果表明唾液乳杆菌可以显著延长秀丽线虫寿命,寿命延长率为11.9%(p<0.05),且延缓衰老作用不是由于唾液乳杆菌菌株与标准菌株大肠杆菌OP50革兰氏分类的差异,并且与菌株的生长氧需求和对宿主的毒性无关。此外,饲喂唾液乳杆菌在延长线虫寿命的同时显著影响线虫的摄食及生长情况,显著提高线虫的呼吸强度及体内SOD活性(p<0.05),表明唾液乳杆菌的抗衰老活性可能是通过能量限制效应而达到的。研究结果为唾液乳杆菌抗衰老研究提供了新的证据,为抗衰老益生菌产品的开发奠定了基础。

Abstract: In this study,we studied anti-aging effects of Lactobacillus salivarius using C. elegans as model,and the anti-aging mechanism of Lactobacillus salivarius was also clarified. The results showed that feeding Lactobacillus salivarius significantly extended the mean life span of C. elegans by up to 11.9%(p <0.05),compared to that of control nematodes. The reduced pharyngeal pumping rate,growth,and increased superoxide dismutase(SOD) activity and XTT reduction capacity were also observed in Lactobacillus salivarius feeding worms(p<0.05),indicating that anti-aging activity of Lactobacillus salivarius may be associated with energy restriction. Our study provided the direct evidence for the anti-aging function of Lactobacillus salivarius,and formed the foundation for developing the anti-aging probiotics.