HPLC法同时测定植物油中维生素A和不同构型维生素E含量

姜波; 胡文忠; 刘长建; 姜爱丽; 王艳颖; 高琳

doi:10.13386/j.issn1002-0306.2015.03.059

HPLC法同时测定植物油中维生素A和不同构型维生素E含量

Simultaneous determination of vitamin A and different configuration of vitamin E in vegetable oils by HPLC

摘要

摘要: 以石油醚为溶剂,用索氏提取法提取了黑芝麻、白花生、黑花生、葵花籽、大豆、棉花籽的油脂。油样品先用正己烷溶解,然后用甲醇定容,其中正己烷含量为20%（v/v）,用Hypersil ODS2 5μm色谱柱,甲醇为流动相,对所提油脂中维生素A、α-维生素E、γ-（β）维生素E、δ-维生素E含量进行检测。实验结果表明,在204nm波长下,维生素A、α-维生素E、γ-（β）维生素E、δ-维生素E在0²00μg/m L范围内与峰面积有良好的线性关系,标准曲线相关系数r均大于0.9978。维生素A、α-维生素E、γ-（β）维生素E、δ-维生素E加标平均回收率分别为97.83%、98.58%、98.75%、99.93%。

Abstract: Oils were extracted from white semen sesami nigrum,white peanut,black peanut,sunflower seed,soybean and cotton seed by Soxhlet extraction with mineral ether.Oil samples were dissolved with normal hexane and being a constant volume with methyl alcohol,the content of normal hexane was 20%( v / v) in the solution.The contents of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E in the extracted oils were determined on a ODS2 column,using methyl alcohol as mobile phase. Experimental results showed that linear relations of the concentrations of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E to peak areas were obtained within0~200μg / m L at 204 nm. The correlation coefficients were more than 0.9978. The average recoveries of vitamin A,vitamin α- E,vitamin γ-( β) E,vitamin δ- E were 97.83%,98.58%,98.75% and 99.93%,respectively.