牦牛乳酪蛋白降血压肽制备工艺及分离纯化研究

Study of preparation and purification process of antihypertensive peptides from Yak milk casein

摘要: 研究了胰蛋白酶制备降血压肽的最佳水解条件和分离纯化工艺。通过单因素、正交实验,确定了最佳水解条件胰蛋白酶添加量2.5%,温度37℃,最适p H8.0,反应时间2.5h,水解产物抑制血管紧张素转化酶的能力为85.26%;通过液相色谱及质朴检测,得到降血压肽的序列片段为HQGLPQEVLNENLLR、AVPYPQR和TKVIPYVR。

Abstract: To prepare the antihypertensive peptides,the optimal enzymatic hydrolysis conditions of Trypsin and purification process were studied.The optimal hydrolysis conditions were determined by single factors and orthogonal experiments,and the optimum hydrolysis conditions were temperature 37℃,p H8.0,trypsin added2.5%,with reaction 2.5h,the capacity of angiotensin- converting enzyme inhibition was 85.26%. Hydrolyzates were separated and purified by high performance liquid chromatography( HPLC) and mass spectrometry,and the amino acid sequences were HQGLPQEVLNENLLR,AVPYPQR and TKVIPYVR.