低分子量罗非鱼眼透明质酸的制备及其抗氧化性研究

高瑞昌; 陈辉; 李来好; 于刚; 吴燕燕; 胡晓; 陈胜军

doi:10.13386/j.issn1002-0306.2015.03.003

低分子量罗非鱼眼透明质酸的制备及其抗氧化性研究

Preparation and antioxidant properties of low molecular weigh hyaluronic acid from tilapia eye

摘要

摘要: 本研究采用微波结合过氧化氢+抗坏血酸法降解罗非鱼眼透明质酸,通过改变降解条件制备相对分子量分别为小于5、5¹0、10³0、30⁶0ku的低分子量透明质酸（LMWHA）。研究比较了不同分子量LMWHA对·OH、DPPH自由基的清除作用以及还原力的大小。结果显示,随着LMWHA分子量的降低其对DPPH的清除率以及还原力增大,而对·OH的清除率减小,这种现象可能与LMWHA清除自由基的作用机理有关,LMWHA清除DPPH自由基以及还原力的大小依赖于其双键的数量,而清除·OH能力的大小则依赖于LMWHA链上羟基以及其螯合过渡金属离子的能力。实验证明低分子量透明质酸具有良好的抗氧化性。

Abstract: LMWHA with relative molecular weight less than 5,5~10,10~30,30~60ku were prepared by changing the degradation conditions of the microwave combined with hydrogen peroxide and ascorbic acid. Reducing power,scavenging ability on ·OH and DPPH free radical of different molecular weight LMWHA were determined. Results showed that the scavenging ability of DPPH free radical and reducing power increased with molecular weight decreased,while the scavenging ability of ·OH decreased,this phenomenon may be associated with the mechanism of LMWHA removing free radicals,the scavenging ability of DPPH and reducing power of LMWHA depended on the number of the double bonds,but the scavenging ability of ·OH depended on the Hydroxyl groups of LMWHA and the ability of chelating transition metal ions.Therefore,low molecular weight hyaluronic acid had good oxidation resistance.