黑小麦麸皮戊聚糖的酶法提取工艺条件优化与理化性质分析

孙元琳; 仪鑫; 王锋; 宋昱; 张陇清

doi:10.13386/j.issn1002-0306.2015.02.031

黑小麦麸皮戊聚糖的酶法提取工艺条件优化与理化性质分析

Optimization of enzymatic extraction process and physicochemical properties of pentosans from black-grained wheat bran

摘要

摘要: 研究了黑小麦麸皮戊聚糖的酶法提取工艺条件,并对其单糖组成和相对分子质量等理化性质进行分析。运用单因素实验方法,分析了提取工艺中料液比、加酶量、提取温度、溶液p H、提取时间对戊聚糖提取率的影响,并通过正交实验对其工艺条件进行优化,确定了酶法提取戊聚糖的最佳工艺参数为:加酶量12mg/10g底物、提取温度60℃、提取时间90min、溶液p H6、料液比1∶10(g/m L)。在此条件下,戊聚糖的提取率为4.17%。通过气相色谱(GC)和高效液相色谱(HPLC)分析了黑小麦麸皮酶提戊聚糖EEP的理化性质。结果表明,EEP主要由阿拉伯糖(Ara)、木糖(Xyl)和葡萄糖(Glc)组成,并含有微量半乳糖(Gal),其摩尔比为Ara∶Xyl∶Glc=1.0∶1.55∶3.19,Ara/Xyl比值为0.64。EEP的相对分子质量为2.16×104。

Abstract: To study the enzymatic extraction technique and the physicochemical properties of pentosans from black-grained wheat bran. The effect of xylanase quantity, extraction temperature, p H, time duration, ratio of material to liquid on the yield of pentosan were studied by single-factor experiments and the technology parameters were optimized by orthogonal experiment. The optimized parameters were:extracting for 90 min at60℃, p H6, with pentosanase quantity of 12mg/10 g substrates and 1 ∶10 ratio of material to liquid. Under the optimum conditions, the yield of EEP was 4.17%. Physicochemical properties of EEP were analyzed using GC, and HPLC instruments. The results showed that EEP was composed mainly of arabinose, xylose, glucose, and trace amount of galactose, mole ratio of Ara∶Xyl∶Glc=1.0∶1.55∶3.19. The Ara/Xyl value was 0.64. It had a relative low Mw of 2.16×104.