黑米花色苷的分离纯化及其抗氧化性的研究

王巧娥; 谢丹; 钱洁; 任海毅; 董银卯

doi:10.13386/j.issn1002-0306.2015.02.025

黑米花色苷的分离纯化及其抗氧化性的研究

Study on separation of anthocyanins from black rice and their antioxidation

摘要

摘要: 对黑米花色苷进行分离纯化并研究不同部分的抗氧化活性。首先采用溶剂萃取的方法将黑米花色苷粗提取物依极性大小分成石油醚可溶部位、乙酸乙酯可溶部位、正丁醇可溶部位和水可溶部位,并对各部位清除2,2-二苯基-1-苦味肼基自由基（DPPH·）和羟基自由基（·OH）的活性进行了分析,确定了抗氧化能力最强的组分是水可溶部分。其次用大孔树脂柱层析的方法对水可溶部位进行了细分,得到C1、C2、C3、C4、C5五个组分,并对各组分清除DPPH·和·OH的活性进行了分析,确定了各组分抗氧化能力从大到小依次为:C2>C3>C4>C1>C5。研究发现,黑米花色苷水可溶部位中20%⁴0%乙醇洗脱的部分（C2、C3）具有更强的抗氧化活性。

Abstract: Anthocyanins from black rice were separated and their antioxidation was evaluated. Anthocyanin crude extract of black rice was separated to petroleum ether part, ethyl acetate part, n-butyl alcohol part and water soluble part by solvent extraction method. The DPPH radical and hydroxyl radical-scavenging activities were used to determine the antioxidant capacity of each part, the water soluble part was confirmed to have the most significant antioxidation. Then it was separated to five fractions which were C1, C2, C3, C4, C5 by macroporous resin column chromatography method and the DPPH radical and hydroxyl radical-scavenging activities of each fraction were analyzed. The results showed that the antioxidant capacity in a descending order as:C2, C3, C4, C1, C5. Among the water soluble part, the fraction eluted by 20%⁴0% ethanol solution had the highest antioxidant capacity.