Abstract: Micro broth dilution method was employed to investigate the minimal inhibitory concentration (MIC) of the grape cane extracts to 3 kinds of fungi and 5 kinds of bacteria. The results showed that the grape cane extracts had significant effect on inhibiting the activities of selected microorganisms. In order to acquire more information about the application of grape cane extracts on table grape storage, compared the preservative effect of three substrates, i.e. grape cane extracts, chitosan, and the mixture of above two substrates on table grape (Vitis vinifera L. cv. Red Globe) . It was found that preservative films smearing with grape cane extracts and chitosan efficiently prevented the clusters from serious dehydration, helped sustain the soluble solid content and titratable acidity of berries, and significantly reduced (p <0.05) the total plate count of fungi and bacteria on the berry surface.