基于响应曲面法优化天麻多酚的提取工艺

Optimization of extraction of phenolic compounds from Gastrodia elata using response surface methodology

摘要: 目的:对天麻总多酚的提取工艺进行优化。方法:以天麻为材料在单因素实验结果的基础上,采用三因素三水平的Box-Behnken响应曲面设计。结果:天麻提取的最佳条件为乙醇浓度57%,提取温度69℃,提取时间53min,该条件下多酚提取含量达44.96mg GAE/g,且提取温度对总多酚的得率影响最大。结论:在最佳条件下天麻多酚得率实际值是预测值的98.48%,接近预测值,可用于天麻多酚的提取。

Abstract: Basis on the results of the single factor experiments,Gastrodia elata was used as materials to optimize the extraction conditions of total phenolics by using response surface methodology involved a three leves,three variable Box- Behnken design.The results showed that the optimum polyphenolics extraction conditions were 57%ethanol as extraction solvent,extraction temperature 69℃ and extraction time 53 min.Under the optimum conditions,the experimental polyphenolics content was 44.96 mg GAE/ g of Gastrodia elata tested,which was well matched with the predicted content.Thus,the optimum conditions can be used to extract the polyphenolics from Gastrodia elata.