河豚鱼皮胶原寡肽制备工艺的优化

Optimization of preparation process of collagen oligopeptide from puffer fish skin

摘要: 研究以无毒河豚鱼皮作为原料,采用两步酶解法制备胶原寡肽的最佳工艺。以水解度和分子量为指标,通过单因素和正交实验优化工艺条件。实验结果表明,第一步最佳用酶为鱼鳞鱼皮胶原蛋白水解专用酶或胰蛋白酶,第二步最佳用酶为酸性蛋白酶。酸性蛋白酶的最佳酶解条件为:投酶量2%,p H4.5,温度50℃,水解时间8h。制备得到的河豚鱼皮胶原寡肽分子量分布主要集中在100~600u。冻干样品的胶原肽含量为94.0%,灰分为0.71%,水分为5.2%。

Abstract: The best preparation process of collagen oligopeptide from puffer fish skin by two- step enzymatic hydrolysis was studied.Taking hydrolysis degree and molecular weight as indices,the hydrolysis conditions were optimized by the single factor experiments and orthogonal tests. The results showed that the first- step optimal enzyme was fish scale collagen hydrolysis special enzyme or trypsin and the second- step optimal enzyme was acid protease.The optimal hydrolysis conditions of acid protease were found to be reaction at 50℃ and p H of 4.5for 8h with protease concentration of 2%.The molecular weight of prepared collagen oligopeptide ranged from 100 u to 600 u.The collagen peptide,ash and water content of the lyophilized sample was respectively 94.0%,0.71%,and5.2%.